Detection of γ-H2AX, a Biomarker for DNA Double-strand Breaks, in Urinary Bladders of N -Butyl- N -(4-Hydroxybutyl)-Nitrosamine-Treated Rats

نویسندگان

  • Takeshi Toyoda
  • Jun-ichi Akagi
  • Young-Man Cho
  • Yasuko Mizuta
  • Saeko Onami
  • Isamu Suzuki
  • Kumiko Ogawa
چکیده

To evaluate the potential role of DNA repair in bladder carcinogenesis, we performed an immunohistochemical analysis of expression of various DNA repair enzymes and γ-H2AX, a high-sensitivity marker of DNA double-strand breaks, in the urothelium of male F344 rats treated with N-butyl-N-(4-hydroxybutyl)-nitrosamine (BBN), a bladder-specific carcinogen. Our results clearly demonstrated that γ-H2AX aggregation was specifically generated in nuclei of bladder epithelial cells of BBN-treated rats, which was not found in untreated controls or mesenchymal cells. γ-H2AX-positive cells were detected not only in hyperplastic and neoplastic areas but also in the normal-like urothelium after BBN treatment. These data indicate that γ-H2AX has potential as a useful biomarker for early detection of genotoxicity in the rat urinary bladder. To the best of our knowledge, this is the first report demonstrating expression of γ-H2AX during bladder carcinogenesis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluating Gamma-H2AX Expression as a Biomarker of DNA Damage after X-ray in Angiography Patients

Objective: Coronary heart disease (CHD) is one of the most common diseases. Coronary angiography (CAG) is an important apparatus used to diagnose and treat this disease. Since angiography is performed through exposure to ionizing radiation, it can cause harmful effects induced by double-stranded breaks in DNA which is potentially life-threatening damage. The aim of the present study is to inves...

متن کامل

γ-H2AX as a protein biomarker for radiation exposure response in ductal carcinoma breast tumors: Experimental evidence and literature review

Background: H2AX is a histone variant that is systematically found and ubiquitously distributed throughout the genome. DNA double-strand breaks (DSBs) induce phosphorylation of H2AX at serine 139 (γH2AX), an immunocytochemical assay with antibodies recognizing γH2AX has become the gold standard for the detection of DSBs. The importance of this assay to investigate different individu...

متن کامل

Residual DNA double strand breaks correlates with excess acute toxicity from radiotherapy

Introduction: A high risk for development of severe side effects after radiotherapy may be correlated with high cellular radiosensitivity. To enhance radiation therapy efficiency a fast and reliable in-vitro test is desirable to identify radiosensitive patients. The aim of present study was to identify the mechanism of radiation induced DNA double-strand breaks (DSBs) and DSB r...

متن کامل

Lack of enhancing effect of mucosal regeneration following ulceration of the urinary bladder on N-butyl-N-(4-hydroxybutyl)nitrosamine carcinogenesis in rats.

The potential enhancing effects of the regeneration response of the urothelium following ulceration of the bladder mucosa on N-butyl-N-(4-hydroxybutyl)nitrosamine carcinogenesis were examined in male Fischer rats. The carcinogen was administered in the drinking water at a concentration of 0.05% for 2 weeks. Ulceration was performed by a freezing technique 24 hr before the administration or 24 h...

متن کامل

Gold nanoparticles can induce more apoptosis and double strand breaks on HT-29 cells irradiated by 18 MV photons

Introduction: To increase therapeutic ratio, using radio-sensitizers is recommended and nowadays some types of them are utilized clinically. In recent years, Gold nanoparticles (GNPs) were considered as radio-sensitizers in many studies due to high atomic number. Although theoretical works showed that GNPs have an insignificant effect at MV energies, some In-vitro and In-vivo s...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 26  شماره 

صفحات  -

تاریخ انتشار 2013